RNAseq of Plasmodium falciparum with loxP flanked bromodomain in PfGC... - SRA

SRX22603728: RNAseq of Plasmodium falciparum with loxP flanked bromodomain in PfGCN5 excised by induction of diCre by rapamycin or left intact in dmso control at different hours post invasion
1 ILLUMINA (Illumina NovaSeq 6000) run: 12.7M spots, 3.7G bases, 1.2Gb downloads

Design: mRNA was purified from DNase treated total RNA by oligo d(T) purification and Indexed mRNA libraries were prepared using the Illumina Truseq mRNA library prep and libraries were 150 bp paired-end sequenced on the Illumina Novaseq 6000 system at the Victorian Clinical Genetics Services (VCGS), Murdochs Children Research Institute (MCRI), Parkville, Victoria, Australia.

Submitted by: The University of Melbourne

Study: Plasmodium falciparum GCN5-TY1 ChIPseq and GCN5 knockout RNAseq and Pf H2B.Zac ChIPseq

PRJNA1043853 • SRP473451 • All experiments • All runs

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The malaria parasite P. falciparum regulates gene expression and chromatin structure through modifying the histones within nucleosomes. This study analysed the histone acetyl transferase PfGCN5 by determining its sites of enrichment within the genome and the effect on gene expression and acetylation of the histone Pf H2B.Z when PfGCN5 was knocked-out. PfGCN5 is essential for parasite survival and is a potential drug target and this study provided information on PfGCN5 function relevant to its investigation as a drug target.

Sample:

SAMN38354817 • SRS19609747 • All experiments • All runs

Organism: Plasmodium falciparum NF54

Library:

Name: GCN5_loxP_rapa_10h_RNAseq_rep2_lib

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Oligo-dT

Layout: PAIRED

Runs: 1 run, 12.7M spots, 3.7G bases, 1.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR26909698	12,708,994	3.7G	1.2Gb	2023-12-31

ID:: 30606174

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